Efek Antibakteri Kombinasi Daun Teh Hijau (Camellia sinensis) dengan Amoksisilin pada Staphylococcus aureus atau Escherichia coli secara in vitro

Abstract

Introduction: Green tea leaf (C.sinensis) can be used as a treatment of bacterial infection because it contains of anti-bacterial compounds. Antibiotics resistance is caused by using antibiotics that does not suit with indication, and does not suit with the dose. This study was purposed to define the anti-microbial activity of amoxcicillin combined with C. sinensis leaf (methanolic extract,decoction) against S. aureus and E. coli.

Method: The antibacterial activities (single,combined) low dose were measured through zone of inhibition (ZOI) test with agar well diffusion method. The result of the ZOI combinations were interpreted with the Ameri-Ziaei Double Antibiotic Synergism Test (AZDAST). The data was analysed with Kolmogorov-Smirnov, followed by a non-parametric test Mann-Whitney Test, with the value of significance on  p<0.05.

Results: Combination of Methanolic extract of C. sinensis leaf with Amoxcicillin (EMCA) and combination of Decoction of C. sinensis leaf with Amoxcicillin (DCA) defined on S. aureus (p<0.05) I.e 12.3 ± 2.9 mm and 21.6 ± 1.1 mm , respectively, were compared to amoxcicillin low dose (5.7±4.93), EMC high and low doses (9.7±0.57 mm;8.3±0.57 mm) and DC all doses (0 mm). DCA combination defined on E.coli while the EMCA combination did not define on E.coli

Conclusion: Low dose EMCA combination on S.aureus is synergistics interaction but in DCA is antagonistics interaction. EMCA and DCA combination are not distinguishable interaction except in DCA (DC+A+;DC-A+) on E.coli

Keywords: Camellia sinensis, Amoxcicillin, antibiotic resistance, S.aureus, E.coli