Pengaruh Suplementasi Minyak Lalat Tentara Hitam (Hermetia illucens L.) Terproteksi Metaloenzim Zink dan Selenium Terhadap Diferensiasi Leukosit Ayam Petelur

Rodinatul Munawaroh, Sama' Iradat Tito, Brahmadhita Pratama Mahardhika

Abstract


This study aimed to examine the effects of black soldier fly (Hermetia illucens L.) oil protected with zinc and selenium metalloenzymes on leukocyte differentiation in laying hens. The design used in this study was a Completely Randomized Factorial Design (CRFD) with two treatment factors and three replications. The experimental design used was a Completely Randomized Factorial Design (CRFD) with two treatment factors and three replications. The factors consisted of black soldier fly oil dosage (0%, 1.5%, and 3%) and antioxidant type (zinc, selenium, and a combination of zinc and selenium). A total of 27 ISA Brown laying hens were fed the experimental diets for 30 days. Data were analyzed using Analysis of Variance (ANOVA), followed by Duncan’s multiple range test when significant differences were observed. Leukocyte differentiation parameters included heterophils, lymphocytes, monocytes, eosinophils, basophils, and the heterophil-to-lymphocyte (H/L) ratio. The results showed that black soldier fly oil supplementation maintained leukocyte differentiation within normal physiological ranges. Supplementation with 3% BSF oil significantly increased heterophils and the H/L ratio (P<0.05) while decreasing lymphocytes, indicating an elevated stress response. All black soldier fly oil doses significantly increased eosinophil levels (P<0.01). Single selenium supplementation resulted in the most favorable leukocyte profile, characterized by the lowest eosinophil levels and stable lymphocyte values. A highly significant interaction (P<0.01) between BSF oil and antioxidant type was observed for eosinophils and the H/L ratio. The optimal formulation was 1.5% BSF oil combined with single selenium supplementation, which provided immunomodulatory effects without inducing excessive stress, supporting sustainable waste utilization for animal feed.

 

Keywords: Hermentia Oil, Zinc, Selenium, Laying Hens, Leukocyte differentiation

ABSTRAK


 

Penelitian ini bertujuan untuk mengkaji pengaruh minyak lalat tentara hitam ( Hermetia illucens L.) terhadap perlindungan metaloenzim zink dan selenium terhadap diferensiasi leukosit ayam petelur. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap Faktorial (RALF) dengan 2 faktor perlakuan dan 3 ulangan. Faktor penelitian yang digunakan yaitu dosis minyak lalat tentara hitam (0%, 1,5%, 3%), dan faktor jenis antioksidan (zink, selenium, serta kombinasi zink+selenium). Sebanyak 27 ekor ayam petelur ISA Brown diberi pakan perlakuan selama 30 hari. Analisis data menggunakan Anayisis Of Varience (ANOVA) dan jika diperoleh data yang berbeda nyata maka dilanjutkan dengan uji Duncan. Parameter yang diuji dari penelitian ini adalah diferensiasi leukosit. Sampel darah dihitung untuk menghitung persentase heterofil, limfosit, monosit, eosinofil, basofil, dan rasio heterofil/limfosit (H/L). Hasil penelitian menunjukkan bahwa pemberian suplementasi minyak lalat tentara hitam dapat mempertahankan rata-rata diferensiasi leukosit dalam kondisi normal. Hasil menunjukkan minyak lalat tentara hitam 3% meningkatkan heterofil dan rasio H/L (P<0,05) serta menurunkan limfosit, menunjukkan peningkatan respon stres. Semua dosis minyak BSF meningkatkan eosinofil secara sangat nyata (P<0,01). Suplementasi selenium tunggal menghasilkan profil leukosit paling menguntungkan dengan eosinofil terendah dan kestabilan limfosit. Interaksi sangat nyata (P<0,01) antara minyak BSF dan antioksidan terjadi pada eosinofil dan rasio H/L. Formulasi optimal adalah minyak BSF dosis 1,5% dengan selenium tunggal, yang memberikan efek imunomodulasi tanpa memicu stres berlebihan, mendukung pemanfaatan limbah untuk pakan berkelanjutan.

 

Kata kunci: Hermetia illucens, Zink, Selenium, Ayam Petelur, Diferensiasi leukosit



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DOI: http://dx.doi.org/10.33474/jimsum.v4i1.30149

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