Pengaruh Kombinasi Dekokta Zingiber officinale var rubrum dan Imperata cylindrica terhadap Kadar TNF-α Serum Tikus Osteoartritis

Abstract

ABSTRACT

Introduction: Osteoarthritis (OA) is a chronic and degenerative joint disease due to damage of cartilage in the joints.  Joint damage in OA patients occurs due to an increase in proinflammatory cytokines, tumor necrosis factor alpha (TNF-α) is a major pro-inflammatory cytokine that plays a role in the pathogenesis of inflammation in OA. Current OA drugs have side effects if are used long term. Therefore, alternative treatments are needed. Red ginger (Zingiber officinale var rubrum) and cogon grass (Imperata cylindrica) have anti-inflammation and antioxidant effects. This research aimed to know the potential of the combination of RGCG  as OA therapy by measuring serum TNF-α of OA rats.

Methods: Male Wistar rats aged 4 months divided into 5 groups (N=5), namely negative control group (KN), positive control group (KP), first treatment with dose 9+40 mg/rat/day (P1), second treatment with dose 18+40 mg/rat/day (P2), and third treatment with dose 36+40 mg/rat/day (P3). Rats were induced with Complete Freund’s Adjuvant (CFA) 0.1 ml/rat intraarticular on the right knee. The administration of the combination of RGCG is given for 10 days. Rats were dissected then blood was taken in the rat's heart, the blood was stored then put in a centrifuge to get blood serum. The serum TNF-α level was assessed by TNF-α ELISA Kit. Data analyzed using One Way ANOVA followed by the Post Hoc test. The result is significant if p<0.05.

Result: Combination of RGCG at dose (0,28 ± 0,01), second treatment dose 18+40 mg/rat/day (0,28 ± 0,01) and third treatment dose 36+40 mg/rat/day (0,27 ± 0,01) are able to decrease the serum TNF-α level significantly (p<0,05) compared with KP, without dependent dose effect.

Conclusion: The given doses of the combination of Red Ginger Rhizome and Cogon Grass dose 9+40 mg/rat/day (T1), 18+40 mg/rat/day (T2) and 36+40 mg/rat/day (T3) are able to decrease the serum TNF-α level of OA rats. 

Keywords: Osteoarthritis, Red Ginger, Cogon Grass, TNF-α.