Efek Kombinasi Jahe Emprit (Zingiber Officinale Var. Amarum) dengan Antibiotik Amoxicillin, Chloramphenicol, Cotrimoxazole terhadap Daya Hambat Pertumbuhan S. Aureus atau E. Coli secara in Vitro

Abstract

Introduction: Inappropriate use of antibiotics in infectious patients can lead to antibiotic resistance. To prevent antibiotic resistance can use antibiotic and herbal alternatives. This study aims to determine the effect of the combination of amoxicillin (AMX), chloramphenicol (CLO), and cotrimoxazole (COT) antibiotics with Ginger Emprit extract (Zingiber officinale var. Amarum) on growth inhibitory of S. aureus and E. coli bacteria.

Method: The Zone of Inhibition (ZOI) test use AZDAST (Ameri-Ziaei Double Antibiotic Synergism Test) method with well modification. Interpretation of ZOI results was carried out by measuring the diameter of the clear zone. The Minimum Inhibitory Test (MIC) was carried out by microdilution using 96-well plates and microdilution using a test tube. Microdilution test was observed using a spectrophotometer, while the macrodilution test observed turbidity and the presence of white sediment on the bottom of the tube. The Minimum Bactericidal Concentration Test (MBC) is a continuation of the MIC test by looking at bacterial growth in nutrient agar.

Results and Discussion: The ZOI test in combination of jahe emprit with AMX, CLO and COT on E. coli and S. aureus is antagonistic. The FICI results of the combination MIC with jahe emprit with AMX and COT on E. coli are additive and the combination with CLO is antagonistic, while combination with AMX, CLO and COT in S.Aureus is additive. The FBCI results from MIC combination of Ginger Emprit with AMX, CLO and COT in E. coli are additive, whereas the combination of AMX and CLO in S. aureus is synergistic.

Conclusion: The results obtained from the combination of jahe emprit with antibiotic cotrimoxazole on S. aureus bacteria had the best result with synergistic results on the MBC experiment.

Keywords: Jahe Emprit, Amoxicillin, Chloramphenicol, Cotrimoxazole, MIC, MBC, S.aureus, E. coli, Combination of Herbal Antibiotics