PENGARUH FREKUENSI STRES FISIK DUA HARI SEKALI TERHADAP KADAR MALONDIALDEHID (MDA) DAN SUPEROKSIDA DISMUTASE (SOD) JARINGAN JANTUNG TIKUS BETINA

Abstract

Introduction: Excessive physical activity can increased heart burden and triggers oxidative stress. Previous research suggestes that physical stress increased levels of endogenous antioxidants. However some studies were disapproving. Therefore, this study aims to determine the effect of various frequency of physical stress on heart levels of MDA and SOD in female subjects.

Methods: Female rats aged 11 weeks were divided into 4 groups (n=6 each), Negative Control (KN), and various intervals of physical stress (Daily/KSH, alternate/KDH and twice weekly/KSK). Physical stress were administered for 4 weeks by means of force swimming for 5 minutes without adaptation. At the end of treatment, all rats were sacrificed and heart were collected and then homogenized. Heart MDA levels were measured using the thiobarbituric acid test method, while heart SOD levels were measured using spectrophotometric methods. Results were analyzed used One Way ANOVA and significance value is set at <0.05.

Result: There is a significant difference in heart MDA levels between KN, KSH, KDH, and KSK were 1103.58 ± 88.33, 885.25 ± 91.22, 864 ± 68.23, 1097.75 ± 115.46, respectively and were significantly different. Heart SOD levels were 8.39 ± 3.3, 4.89 ± 2.08, 13.2 ± 4.15, 4.43 ± 2.74 for KN, KSH, KDH and KSK respectively with significant difference between KN with KSK, KDH and KDH with KSH, KSK. Correlation analyses between MDA and SOD levels shows -0.180 (p=0.399) suggesting a no correlation study.

Conclusion: Various frequency of physical stress increases SOD levels and decreases MDA levels in female rats especially with alternate frequency.