PENGARUH KOMBINASI EKSTRAK AIR DAUN JATI BELANDA, KEMUNING, MURBEI, DAN RIMPANG BANGLE TERHADAP KADAR TNF-α SERUM TIKUS DENGAN DIET TINGGI LEMAK

Abstract

Introduction: Hyperlipidemia lead to atherosclerosis. Bay Cedar (Guazuma ulmifolia), Orange Jessamine (Murraya paniculata), White Mulberry (Morus alba) leaves and Mountain Ginger (Zingiber cassumunar) rhizomes (BOWM) has a potency as antihyperlipidemic drug. This study aims to determine the effect aqueous extract of BOWM on the levels of Tumor Necrosis Factor-α (TNF-α) serum of male wistar rats induced by a modification of an high fat diet (MHFD).

Methods: This study used a laboratory experimental method in vivo with control group posttest only design. 25 male wistar rats, 8 weeks, body weight 150-200 grams were grouped into five, namely Negative Control (KN), Positive Control (KP), Dosage Group 1 (KD1), Dosage Group 2 (KD2), Dosage Group 3 (KD3). The aqueous extract of BOWM was given for 12 weeks along with MHFD treatment. The dose of aqueous extract of BOWM; KD1 (189mg/200gBB), KD2 (378mg/200gBB), KD3 (756mg/200gBB) were given everyday orally. Serum TNF-α levels were measured by TNF-α rat ELISA kit. Data were analyzed by Kruskal Wallis and Mann-Whitney.

Results: The TNF-α serum levels were: KN 280,52 ± 27,28; KP 282,52 ± 45,14; KD1 269,90 ± 7,01; KD2 308,25 ± 30,72; KD3 312,54 ± 33,21. MHFD did not significantly increase serum TNF-α level of KP compared to KN. The aqueous extract of BOWM did not significantly (p>0,05) decrease serum TNF-α level of KD1 compared to KN and KP. Serum TNF-α level of KD3 increased significantly (p<0,05) compared to KN; KD2 and KD3 compared to KD1.

Conclusion: BOWM aqueous extract has no effect on the levels of serum TNF-α in MHFD-induced rat.