PENGARUH FREKUENSI STRES FISIK PADA KADAR TRIGLISERIDA SERUM DAN NONALCOHOLIC FATTY LIVER DISEASE ACTIVITY SCORE (NAS) TIKUS WISTAR BETINA

Abstract

Introduction: Excessive physical exercise can cause physical stress and an increase in serum triglyseride/TG, free fatty acid (FFA) and the occurrence of Non-Alcoholic Liver Disease (NASH). Therefore, this research aim to determine the role of various interval of physical activity to determine possible physical stress and its role on serum Tlevels and NASH activity score (NAS).

Methods: Female Wistar rats aged 5 weeks were divided into 5 groups, namely Pre-treatment normal control (KNPP, n = 6), Negative control (KN, n = 6), and 3 groups of various intervals of physical stress (Daily/KSH (n = 6), Alternate/KDH (n = 6) and twice weekly/KSK (n = 6)). Rats were subjected to force swimming without adaptation for 5 minutes in 4 weeks the culled to collect serum and liver tissue. Serum TG levels were measured using spectrophotometry and NAS was calculated using Image J on liver histology preparations. Data were analysed using One-way ANOVA followed by post hoc LSD and a Pearson correlation with p are set at < 0.05.

Results: Statistical analysis showed no significant differences (p> 0.05) between serum TG levels in all groups although on a Post Hoc LSD test shown significant difference between KN (101.17+70.22) with KDH (181.33+88.50) (p = 0.039) and KDH (181.33+88.50) with KSH (84.50 + 40.80) (p = 0.015). NAS score analyses shows no significant difference between groups on one-way ANOVA or post-hoc LSD or Pearson correlation analyses between serum TG level and NAS.

Conclusion: Various intervals of physical stress for 4 weeks did not affect serum TG levels nor NAS in the liver of female wistar rats fed normal diet suggesting that 5 minutes of force swim physical stress is well tolerated by the subjects when daily diet is normal.