PENGARUH FREKUENSI STRES FISIK PADA KADAR ASPARTATE AMINOTRANSFERASE (AST) DAN STRUKTUR HISTOLOGIS JANTUNG TIKUS BETINA DENGAN DIET ATHEROGENIK

Abstract

Introduction: Atherogenic diet can cause hyperlipidemia, leading to elevated serum Aspartate Aminotransferase (AST) levels and changes of the heart structures. Excessive physical stress can disrupt body’s metabolism. However, some studies suggest physical stress were beneficial during hyperlipidemia. This study aims to determine the role of various frequency of physical stress on AST levels and histological structure of the heart during atherogenic diet induced hyperlipidemia.

Methods: Female wistar rats were divided into 6 groups, (KN, KPP, KP, KSK, KDH, and KSH, n=5, in each groups). KN were fed with standard diet for 10 weeks, KPP and KP were fed with atherogenic diet for 6 and 10 weeks respectively. KSK, KDH, and KSH were fed with atherogenic for 10 weeks with treatment of physical stress twice a week, once every two days, and daily, respectively for 4 weeks. Physical stress were conducted by forced swimming for 5 minutes. AST levels were measured using spectrophotometric method, whereas the heart structure were assessed using H&E staining and imaged using trinocular microscope. Images were measured using Fuji Image software and results were analyzed using SPSS and p is set as 0.05.

Results: Atherogenic diet and various frequencies of physical stress has no effect on serum levels of AST. Atherogenic diet increase left ventricular thickness (KN: 42.05 ± 10.8 vs. KP: 45.82 ± 8.53) and decrease number of left cardiomyocytes (KN: 18.11 ± 3.18 vs. KP: 11.62 ± 4.51). Various frequencies of physical stress significantly increase thickness of the right and left ventricles, as well as changing the number of cardiomyocytes.

Conclusion: Atherogenic diet or physical stress has no effect on serum AST levels. Atherogenic diet increase thickness of the heart ventricles while various frequencies of physical stress affe. While the various frequencies of physical stress effect of ventricular thickness and the number of the heart muscle cells on both side.