EFEK SARI BIJI KEDELAI (Glycine max), RIMPANG JAHE (Zingiber officinale) DAN KOMBINASINYA TERHADAP KADAR SUPEROXIDE DISMUTASE (SOD) SERUM DAN DENSITAS ENDOTEL AORTA TIKUS MODEL DIABETES

Abstract

Introduction: Hyperglycemic on diabetes melitus contribute to increase vasculopathy diabetic complication. Soya seed and gingger rhizome have been known as antidiabetic effect, but its combination has not been evaluated. This research aimed to know about potential of soya seed (Glycine max), gingger rhizome (Zingiber officinale) extract and its combination on serum SOD level and aortic endothelial density in diabetic rat model.

Method: This study used Sprague dawley male rats 3 months old, which were divided into 2 control groups (normal control and treatment control groups) and 4 treatment groups (soya, gingger, combination delja I and delja II groups) (n=5 rats). The rats were induced on a high-fat-fructose diet (HFFD) and streptozotocin (STZ). Subsequently, soyaseed 5 g/kgBW, ginger rhizome extract 500 mg/kgBW, combination of delja I and delja II were given orally for 30 days. Serum SOD levels were measured by using microplate reader λ = 450 nm and aortic endothelial density using microscop and ImageJ. Data analyzed by using SPSS by significance p <0.05.

Result : Soya seed extract, ginger rhizome and delja II combination significantly increase serum SOD levels approximately 2 times, 2 times and 3 times, and increase aortic endothelial density approximately 60%, 80 % and 100% respectively, compared by treatment control group (p <0.05). The induction of DTLF and STZ in the treatment control group increase serum SOD levels and aortic endothelial density 70% and 60% compared by normal control group .

Conclusion: According to the result above, soya seed extract, ginger rhizome and delja II combination can inhibit reduction of serum SOD levels and aortic endothelial density in diabetic rat model.