Efek Kombinasi Sambiloto, Daun Salam, Kayu Manis, dan Temulawak terhadap Kadar Superoxide Dismutase (SOD) dan Malondialdehyde (MDA) Serum Tikus Model Hiperglikemia

Abstract

Introduction: Chronic hyperglycemia caused diabetes mellitus (DM). Flavonoids are the main active ingredients which have a combination of sambiloto leaves, salam leaves, cinnamon, and temulawak which have an antioxidant effects. The purpose of this study was to proved the antioxidant effects on increased of superoxide dismutase (SOD) and decreased of malondialdehyde (MDA) serum levels.

Method: The study design was in vivo laboratory experimental, using a control group post test only design in 2-3 months old male wistar (Rattus novergicus). The subjects were divided into hyperglycemia groups (KP, T1: 250 mg/kgBW, T2: 250 mg/kgBW, and T3: 500 mg/kgBW) and negative control groups (KN). Rats were adapted for 7 days and then induced low-dose streptozotocin (STZ) (25 mg/kgBW) and 10% High Fructose Diet (HFD). The combination of sambiloto leaves, salam leaves, cinnamon, and temulawak is administered per stomach sonde for 30 days after the condition of hyperglycemia (GDP ≥126 g/dl). Examination of SOD serum levels using a spectrophotometer λ 580 nm and MDA serum levels using a spectrophotometer λ 532.8 nm. Data analysis using One-Way ANOVA with significance p <0.05.

Results: Administration of sambiloto leaves, salam leaves, cinnamon, and temulawak in the treatment group (T1, T2, and T3) than KP showed an increase in SOD at a dose of 250 mg/kgBW, while decreasing of MDA occured at a dose of 125 mg/kgBW.

Conclusion: The results of sambiloto leaves, salam leaves, cinnamon, and temulawak combination showed a p value of <0.05 which showed a significant effect on SOD and MDA serum levels in the hyperglycemic rats.