Antibacterial potential of four herbal plants (Syzygium cumini, Piper ornatum, Anredera cordifolia, and Alpinia galangan) against Staphylococcus aureus and Escherichia coli
Abstract
ABSTRACT
Introduction: The lack of new antibiotics and the increasing rate of resistance on pathogens requires the discovery of bioactive compounds with antibacterial activity. Using etnopharmacology knowledge, several Indonesian herbs, in particular Juwet (Syzygium cumini), Sirih merah (Piper ornatum), Binahong (Anredera cordifolia) and Laos (Alpinia galangan) had been shown possess wound-healing, anti-inflammatory and gastroprotective activities. It was postulated that these plants would also have antibacterial activity.
Method: This study aims to assess the potential for antibacterial activity of these plants, in which decoctation, methanolic and choloroform extraction was used against Staphylococcus aureus and Escherichia coli growth by measuring and comparing zone of inhibition (ZOI), minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC).
Results: All plants tested had some activity against S. aureus, but none were found to be active against E. coli. Furthermore, various extraction methods of S. cumini and A. galangan had antibacterial activity against S. aureus with a ZOI of 10 15 mm and 12 25 mm, respectively, with increased antibacterial activity found in non-polar extracts. Methanolic extract of S. cumini acts as a bactericidal at 0.391 mg/ml, whilst other extracts had a bactericidal activity at 6.25 mg/ml.
Conclusion: From four plants tested, methanolic extracts of S. cumini and A. galangan has medium to strong antibacterial activity against clinical S. aureus strains.
Keywords: Juwet, Sirih Merah, Binahong, Laos, Antibacterial, Antibiotic
Full Text:
PDFReferences
The Centers for Disease Control and Prevention (CDC). CDC in Indonesia Factsheet. Centers for Disease Control and Prevention, (CDC) 2013; pp.12.
Azizi, B.H., Zulkifli, H.I. & Kasim, M.S. Protective and risk factors for acute respiratory infections in hospitalized urban Malaysian children: a case control study. Southeast Asian J Trop Med Public Health 1995; 26.
van Gageldonk-Lafeber, A.B., van der Sande, M.A., Hijnen, M.L, Peeters M.F., Bartelds, A.I, Wilbrink B. Risk Factors for Acute Respiratory Tract Infections in General Practitioner Patients in Netherlands: a case control study. BMC Infect Dis 2007; Apr 27;7:35
Chen, C.J. & Huang, Y.C. New epidemiology of Staphylococcus aureus infection in Asia. Clinical Microbiology and Infection 2014; 20(7), pp.605623.
Demain, A.L. & Sanchez, S.. Microbial drug discovery: 80 years of progress. The Journal of antibiotics 2009; 62(1), pp.516.
Hoagland, D.T, Liu J., Lee, R.B., Lee R.E. New agents for the treatment of drug-resistant Mycobacterium tuberculosis. Advanced Drug Delivery Reviews 2016; Jul 1;102:55-72
de Lima Procpio, R.E, Silva I.R., Martins M.K, Azevedo J.L, Araujo, J.M. Antibiotics produced by Streptomyces. The Brazilian Journal of Infectious Diseases 2012; 16(5), pp.466471.
Newman, D. & Cragg, G. Natural Products as Sources of New Drugs over the 30 Years from 1981 to 2010. Journal of Natural Products, 2012; 75(3), pp.311335.
Bettiol, E, Wetherington J.D., Schmitt, N., Harbarth S., COMBACTE consortium. Challenges and solutions for clinical development of new antibacterial agents: Results of a survey among pharmaceutical industry professionals. Antimicrobial Agents and Chemotherapy 2015; 59(7), pp.36953699.
Abdelmohsen, U.R, Grkovic T., Balasubramanian S., Kamel M.S., Quinn R.J., Hentschel U. Elicitation of secondary metabolism in actinomycetes. Biotechnology Advances 2015; 33(6), pp.798811.
Cowan, M.M.. Plant products as antimicrobial agents. Clinical microbiology reviews 1999 12(4), pp.56482.
Gonzlez-Lamothe, R., Mitchell, G., Diarra, M.S., Molouin, F., Bouarab, K. Plant antimicrobial agents and their effects on plant and human pathogens. International Journal of Molecular Sciences 2009; 10(8), pp.34003419.
Hfling, J.F., Anibal P.C., Obando-Pereda, G.A, Peixoto I.A., Furletti V.F., Foglio M.A., Goncalves R.B. Antimicrobial potential of some plant extracts against Candida species. Brazilian journal of biology = Revista brasleira de biologia, 2010.; 70(4), pp.10651068.
Moussaoui, F. & Alaoui, T.. Evaluation of antibacterial activity and synergistic effect between antibiotic and the essential oils of some medicinal plants. Asian Pacific Journal of Tropical Biomedicine 2015; 6(1), pp.3237.
Zaiden, M.R.S., Noor R.A., Badrul A.R., Adlin A., Norazah A., Zakiah I. In vitro Screening of Five Local Medical Plants for Antibacterial Activity Using Disc Diffusion Method. Tropical Biomedicine 2005; 22(2), pp.165170.
Jawetz. E., J.L. Melnick and E.A. Adelberg. Microbiology Untuk Kesehatan. Edisi XVI. , Penertbit Buku Kedokteran EGC. Jakarta, 1986.
Sudarmi, Kadek, Ida Bagus Gede Darmayasa, I Ketut Muksi, Uji fitokima dan daya hambat ekstrak daun juwet (Syzygium cumini) terhadap pertumbuhan Escherichia coli dan Staphylococcus aureus ATCC, Jurnal Simbiosis V September 2017; (2 ): 47 ?51 ISSN: 2337-7224,
Gowri, S. Shyamala dan K. Vasantha, Phytochemical Screening and Antibacterial Activity of Syzygium cumini (L.) (Myrtaceae) Leaves Extracts, International Journal of PharmTech Research, CODEN (USA): IJPRIF 2010, Vol.2, No.2, pp 1569-1573, April-June,
Rani, Beula KR, Sundar SK, Murugan M, Antimicrobial Activity and Phytochemical Study of Medicinal Plant Alpinia Galanga 2016, Vol 9, Issue 3,
Marifah, A. Efek Ekstrak Daun Sirih Merah terhadap Pertumbuhan Bakteri S. aureus, Program Studi Pendidikan Dokter FKIK UIN Syarif Hidayatullah, Jakarta, 2012
Lufika, C., Diana K. Jasaputra ,Djaja Rusmana, Aktivitas Antimikroba Infusa Daun Binahong terhadap S aureus secara in vitro, 2015
Anggun A.W, Uji AKtivitas Antibakteri Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten.) Steenis) Terhadap Bakteri S. aureus Secara In Vitro, 2012.
Agus, R. M., Enny, F., & Dewi, K. Isolasi, Identifikasi serta Uji Aktivitas Antibakteri Senyawa Golongan Triterpenoid dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Staphylococcus aureus dan Escherichia coli, 2013.
Arman, C., & David, P. Aktivitas Antimikroba Daun Binahong Terhadap Pseudomonas aeruginosa dan Staphylococcus aureus yang Sering Menjadi Penyulit pada Penyembuhan Luka Bakar, 2010.
Noorhamdani, A. S., Sudiarto, & Vita, U. Uji Ekstrak Daun Binahong sebagai Antimikroba terhadap Staphylococcus aureus dan Pseudomonas aeruginosa, 2010.
Hasri, Muhammad Anwar, Marwah Karim, Analisis Fenolik dan Daya Hambat Daun Binahong(Anredera cordifolia (ten.) Steenis) , terhadap Eschericia coli dan Staphylococcus aureus, Indonesiam chemistry and application journal, (ICAJ) ISSN : 2549-2314; Volume : 1; Number 1, 2017
Anis Ainurrochmah, Evie Ratnasari, Lisa Lisdiana, Efektivitas Ekstrak Daun Binahong (Anredera cordifolia) terhadap Penghambatan Pertumbuhan Bakteri Shigella flexneri dengan Metode Sumuran, 2013
Hermawan, Anang, Pengaruh Ekstrak Daun Sirih Merah terhadap Pertumbuhan Bakteri S. aureus dan E.coli dengan metode disk, Fakultas Kedokteran Hewan Universitas Airlangga, 2007
Soleha TU, Carolia N, Kurniawan SW, The Inhibition Test of Red Betel Leaves (Piper crocatum) towards Staphylococcus aureus and Salmonella typhi, J MAJORITY Volume 4 Nomor 5, Februari 2015
Ani, U., Dwi, K., & Diah, T. M. Pengaruh Pemberian Ekstrak Daun Binahong Terhadap Kesembuhan Luka Infeksi Staphylococcus aureus pada mencit, 2012
Refbacks
- There are currently no refbacks.
